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Designed to Ensure Compatibility, Quality Control and Quicker Installation!.

Flexible Film (Softwall) Double-tier Isolator Systems


Flexible Film (Softwall) Isolator Systems


CBC Double-Tier Isolator Systems not only help to maximize lab space, but ensure compatibility, quality control, Quicker installation, better traceability & easier reorders.

CBC's Double-Tier isolator Systems come with with all hardware and components required for assembly – saving you time and money compared to jury-rigging a system yourself using parts from different manufacturers and suppliers.

Gnotobiotic islolator systems for rodents.
CBC double-tier flexible film isolator systems help maximize floor space and comes with all the hardware and components necessary for assembly.

Double-Tier Isolator Systems

CBC double-tier, flexible film isolator system maximize limited lab space and come with all hardware and components necessary for assembly.

For gnotobiotic, microbiome, microbial, altered Schaedler flora and other research that requires a controlled environment.

Standard (Positive Pressure) Double-Tier System Sizes:

  • 30" x 2' x2' – Double-Tier/Two isolators.
  • 3' x 2' x 2' – Double-Tier/Two isolators.
  • 4' x 2' x 2' – Double-Tier/Two isolators.
  • 60" W x 30" H x 27" D – EU-Double-Tier/Two isolators.
  • 5' x 2' x 2' – Double-Tier/Two isolators.
  • 6' x 2' x 2' – Double-Tier/Two isolators.
  • Note: All of the above system sizes are also available as negative pressure systems.


What's Included

Double-tier Isolator Systems come with with all hardware and components required for assembly – saving you time and money compared to jury-rigging a system yourself using parts from different manufacturers and suppliers.Note: Also available with and optional motorized adjustable frame for easier accessibility.


Double-Tier Isolator
Schematics/Cut Sheets


3'x2'x2' Double-Tier Schematic/Cut sheet (Model # 50362424122)

4'x2'x2' Double-Tier Schematic/Cut sheet (Model # 50482424122)

5'x2'x2' Double-Tier Schematic/Cut sheet (Model # 50602424122)

Double-Tier (EU) Schematic/Cut sheet


Double-tier softwall isolator systems include:

  • 1 ½" steel powder coated single shelf frame to prevent bending or warping.
  • HDPE tabletop with inserts for attaching isolator components.
  • Two-way locking wheels.
  • 5/8" PVC hold-down rod and SS clamps.
  • Blower and manifold system.
  • (2) 20 mil vinyl isolators.
  • (2) 20 mil urethane floor mat protectors for inside the isolator.
  • (2) 3/8" closed cell foam pad for under isolator.
  • (2) complete 5" diameter inlet filters & (2) complete 5" diameter outlet filters (includes filter media, filter covers, rubber bands and clamps). Note: Lower inlet port and upper outlet port can easily handle warm, moist air. Combined with our standard 5" filters (vs. competitive 3" filters), this design provides greater airflow and improves the isolator's living environment. (see graphic below).
  • (4) 2" neoprene stoppers.
  • (2) Transfer ports (size & type to your specifications)*.
  • (2) Port to isolator neoprene bands and clamps for sealing isolator to transfer port.
  • (2) each of inside and outside transfer port caps.
  • (2) Patent clamps for attaching outside port cap to transfer port.
  • (2) inside rubber band for attaching inside transfer port cap.
  • (2) pairs of 12" diameter/8"glove port CBC glove extensions for better worker dexterity and less stress on isolator. (Note: Available without.)
  • (2) pairs of 15 mil Hypalon/CSM Dry Box Gloves with glove ring kit for attaching gloves to isolator (other types available).
  • (4) 1" neoprene stoppers (other types available).
  • (4) internal racks that can accommodate up to nine mouse cages each.
The CBC Double-tier flexible film starter lab comes with all the lab hardware and accessories needed for researchers to install a fully functional germ free lab that offers complete environmental control for gnotobiotic, microbiome and other research using mice or other rodents. Click here to download flyer.

Download Printable Flyer

Download a printable flyer about the CBC Double-tier Starter Lab.

Download Printable PDF

Download Printable (EU) PDF

Download Printable PDF of Negative Pressure System

CBC' Quality Control Tracking

Each CBC Flexible Film Canopy is Marked With its Own Unique Serial Number .

When you buy one of our gnotobiotic isolator systems or just a canopy, your canopy will be marked with its own unique serial number and manufacture date. Should you ever have a issue with the canopy (or need a replacement) CBC can trace your canopy back to the exact specs previously ordered. The same for replacement canopies for existing frames and filter systems.

  • Traceability.
  • Faster replacement.
  • More accurate replacement.

Each CBC Flexible Film Canopy is Marked With its Own Unique Serial Number and Manufacture Date to Ensure Traceability and Better Customer Support.

CBC glove port glove extensions for better worker dexterity and less stress on isolator.

CBC Glove Port Glove Extensions for Better Worker Dexterity and Less Stress on Isolator

CBC glove extensions (optional) provide better worker dexterity and help reduce stress on isolators at a critical seam areas.  Read more

The CBC Difference

The CBC difference includes our original lap-seam sealing process and the use of 20 mil. polyurethane material that is almost indestructible and helps ensure against contamination due to accidental puncture or tear.

CBC uses a highly durable "lap seam" seal for the majority of our seams, and an even superior "cut/weld/strip seam" seal for high risk areas. Because your reputation, research or production should not risk being compromised!    (See, "Pull Test" and "Puncture Demonstration" videos below.)

The CBC Lap-Seal Seam "Pull" Test

Single Seam Seal vs. CBC Lap Seal Seam  Read more

Because your reputation, research or production should not risk being compromised!
20 mil. Polyurethane Puncture Demonstration

CBC's 20 mil. polyurethane material is almost indestructible and helps ensure against contamination due to accidental puncture or tear.  Read more

Because your reputation, research or production should not risk being compromised!

Improved Living Environment

CBC's lower filter inlet port and upper filter outlet port can more efficiently remove warm, moist air. What's more, our 5" diameter design (vs. competitive 3" diameter) provides greater airflow. The larger filter diameter combined with CBC's unique "inlet/outlet" port design help improve the isolator's living environment.

CBC's lower filter inlet port and upper filter outlet port can more efficiently remove warm, moist air. What's more, our 5 inch diameter design vs. competitive 3 inch diameter provides greater airflow. The larger filter diameter combined with CBC's unique inlet/outlet port design help improve the isolator's living environment.

Accessories and Components:

CBC 9-cage isolator rack for Single- and Double-tier isolator systems.
Internal 9-Cage Isolator Rack

9-cage is standard. 5-cage racks for CBC's Quad isolator system. Custom sizes are available.  Read more

CBC's internal isolator cage racks are easier and faster to assemble!
CBC Patent Clamp for quick attaching transfer port caps.
CBC Patent Clamp

CBC's Patent Clamp for quickly, and securely attaches transfer port caps.  Read more

Patent Clamp with neoprene-gooved band reduces need for vinyl tape
Transfer Sleeves used for isolator to isolator attachments and sterilizing cylinder to isolator connections.
Sterilizing Autoclave Cylinder for water, bedding, cages and other components used in animal research.
Stainless Steel Transfer Carts (Trolleys) with table clamps to secure CBC sterilizing cylinders in place and prevent accidents
Stainless steel, acrylic polypropolene transfer ports.
Transfer port caps with and without flanges.
Transdisk allows you to transfer mice without a transfer sleeve.

Optional Adjustable Frame

For easier accessibility. The motorized adjustable frame goes up or down 30 inches, allowing isolator technicians easier access to the top tier isolator(s).

Tje optional CBC frame goes up or down 30 inches, allowing isolator technicians easier access to the top tier isolator(s).
Original lap-seam seal flexible film, germfree isolator.
Manufacturing high quality, flexible film isolators for universities, researchers and germ-free animal producers since 1978.
CBC Double-tier flexible film isolator system come with all the hardware and components necessary for assembly.

Negative Pressure Double-Tier Isolator System

The CBC 5' x 2' x 2' Negative pressure, Double-Tier isolator helps maximize lab space.

Our Double-tier 5'x 2'x 2' negative pressure, flexible film isolator system comes with all hardware and components necessary for assembly.

CBC Double-tier flexible film, Starter Labs come with all hardware and components necessary for researchers to have a complete working gnotobiotic lab.

Double-Tier Isolator "Starter Labs"

CBC Double-Tier isolators helps maximize lab space.

Our Double-tier flexible film "Starter Labs" come with all hardware and components necessary for researchers to have a complete working gnotobiotic lab.   More Information

Send Us a Message


Class Biologically Clean is a manufacturer of flexible film isolators for gnotobiotic reasearch.

Protecting Both Research and Researchers

Animal Research Gnotobiotic Isolators   |   Bio/Pharmaceutical Barrier & Containment Isolators

608.273.9661 ●
810 Stewart Street, Madison, Wisconsin 53713 ●
info@cbclean.com

Email CBC.
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® CBC is a Registered Trademark of Class Biologically Clean, Ltd.


Flexible Film Isolators, Softwall Cleanrooms, Containment Units and Accessories for Controlled Research Environments.